Regulation of pituitary MT1 melatonin receptor expression by gonadotrophin-releasing hormone (GnRH) and early growth response factor-1 (Egr-1) : in vivo and in vitro studies

Copyright: © 2014 Bae et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This work was funded by the UK Biotechnology and Biological Sciences Research Council (BBSRC; grant BB/F020309/1; http://www.bbsrc.ac.uk/home/home.aspx). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Peer reviewedPublisher PD

Validity and interunit reliability of 10 Hz and 15 Hz GPS units for assessing athlete movement demands

The purpose of this study was to assess the validity and interunit reliability of 10 Hz (Catapult) and 15 Hz (GPSports) Global Positioning System (GPS) units and investigate the differences between these units as measures of team sport athlete movement demands. A team sport simulation circuit was completed by 8 trained male participants. The movement demands examined included: total distance covered (TD), average peak speed, and the distance covered, time spent, and the number of efforts performed low-speed running (0.00-13.99 km·h-1), high-speed running (14.00-19.99 km·h-1), and very highspeed running (>20.00 km·h-1). The degree of difference between the 10 Hz and the 15 Hz GPS units and validity was assessed using a paired samples t-test. Pearson's correlations were also used for validity assessment. Interunit reliability was established using percentage typical error of measurement (%TEM) and intraclass correlations. The findings revealed that 10 Hz GPS units were a valid (p > 0.05) and reliable (%TEM = 1.3%) measure of TD. In contrast, the 15 Hz GPS units exhibited lower validity for TD and average peak speed. Further, as the speed of movement increased the level of error for the 10 Hz and 15 Hz GPS units increased (% TEM = 0.8-19.9). The findings from this study suggest that comparisons should not be undertaken between 10 Hz and 15 Hz GPS units. In general, the 10 Hz GPS units measured movement demands with greater validity and interunit reliability than the 15 Hz units, however, both 10 Hz and 15 Hz units provided the improved measures of movement demands in comparison to 1 Hz and 5 Hz GPS units. © 2014 National Strength and Conditioning Association

The effect of water-based plyometric training on vertical stiffness and athletic performance

© 2018 Sporri et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Since higher vertical stiffness is related to superior athletic performance, training has traditionally been aimed at augmenting this variable to enhance neuromuscular output. However, research has linked elevated stiffness with increased injury risk, therefore, this study examined the effect of a novel training intervention on vertical stiffness and athletic performance. Vertical stiffness, jump performance and athletic performance were assessed in two randomly allocated groups, prior to, and following, an eight-week period. One group was exposed to a training intervention involving aqua-based plyometrics (n = 11) over the 8 weeks while the other acted as a control group (n = 9). The training intervention involved hopping, jumping and bounding in water at a depth of 1.2m whilst control participants performed their normal training. There were no significant changes in vertical stiffness in either group. Countermovement jump height and peak power significantly increased within the aqua plyometric group (p < 0.05). Athletic performance markers improved in the aqua plyometric group as measured using an agility and a 5-bound test exhibiting superior values at the post-test (p < 0.05). The results suggest that an aqua plyometric training program can enhance athletic performance without elevating stiffness. The increase in athletic performance is likely due to a reduction in ground reaction forces created by the buoyancy of the water, causing a shorter amortization phase and a more rapid application of concentric force. The findings from this study can inform exercise professionals and medical staff regarding the ability to enhance neuromuscular performance without elevating vertical stiffness. This has implications for improving athletic performance while concurrently minimising injury risk

Stochastic De-repression of Rhodopsins in Single Photoreceptors of the Fly Retina

The photoreceptors of the Drosophila compound eye are a classical model for studying cell fate specification. Photoreceptors (PRs) are organized in bundles of eight cells with two major types – inner PRs involved in color vision and outer PRs involved in motion detection. In wild type flies, most PRs express a single type of Rhodopsin (Rh): inner PRs express either Rh3, Rh4, Rh5 or Rh6 and outer PRs express Rh1. In outer PRs, the K50 homeodomain protein Dve is a key repressor that acts to ensure exclusive Rh expression. Loss of Dve results in de-repression of Rhodopsins in outer PRs, and leads to a wide distribution of expression levels. To quantify these effects, we introduce an automated image analysis method to measure Rhodopsin levels at the single cell level in 3D confocal stacks. Our sensitive methodology reveals cell-specific differences in Rhodopsin distributions among the outer PRs, observed over a developmental time course. We show that Rhodopsin distributions are consistent with a two-state model of gene expression, in which cells can be in either high or basal states of Rhodopsin production. Our model identifies a significant role of post-transcriptional regulation in establishing the two distinct states. The timescale for interconversion between basal and high states is shown to be on the order of days. Our results indicate that even in the absence of Dve, the Rhodopsin regulatory network can maintain highly stable states. We propose that the role of Dve in outer PRs is to buffer against rare fluctuations in this network

Information effect on voter turnout: How campaign spending mobilises voters

We explore the impact of campaign effort on constituency-level turnout variation in Britain, under the premise that higher levels of campaign visibility stimulate electoral participation. We focus on the relationship between the competitiveness of the race and campaign effort as a provider of electoral information on the one hand, and voter turnout on the other hand. In doing so, we address the role of campaign effort and competitiveness in shaping turnout both independently as well as jointly. Further to this, we seek to add nuance to our understanding of how electoral campaigns mobilise voters by evaluating the comparative ability of different parties – based on whether or not they are ‘viable’ contenders in a particular constituency – to stimulate turnout. We find evidence that campaign effort mobilises voters and has a significant positive effect on voter turnout; this effect is independent from, and unconditioned by, the competitiveness of the race. However, we do find that this effect is mostly driven by the campaign effort of the ‘viable’ contenders in the constituency

Information effect on voter turnout: How campaign spending mobilises voters

We explore the impact of campaign effort on constituency-level turnout variation in Britain, under the premise that higher levels of campaign visibility stimulate electoral participation. We focus on the relationship between the competitiveness of the race and campaign effort as a provider of electoral information on the one hand, and voter turnout on the other hand. In doing so, we address the role of campaign effort and competitiveness in shaping turnout both independently as well as jointly. Further to this, we seek to add nuance to our understanding of how electoral campaigns mobilise voters by evaluating the comparative ability of different parties – based on whether or not they are ‘viable’ contenders in a particular constituency – to stimulate turnout. We find evidence that campaign effort mobilises voters and has a significant positive effect on voter turnout; this effect is independent from, and unconditioned by, the competitiveness of the race. However, we do find that this effect is mostly driven by the campaign effort of the ‘viable’ contenders in the constituency